by Leyi Wang
This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.
- Includes cutting-edge methods and protocols for the study of coronaviruses in domestic animals and poultry
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
File size: 3.9 MB
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